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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exons 4 to 6 have been flanked by loxP sites (size of loxP-flanked region: 1.7 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exons 4-6 should result in loss of function of the Galnt2 gene, by removing the 5' half of the catalytic sub-domain A and by generating a frameshift into downstream exons which generates a premature stop codon.
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The corresponding conditional KO allele is available as line 10264.
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Datamining and Design performed in 2008.
Gene Family:
Transferase Genetic Background:
C57BL/6 Gene Symbol:
Galnt2 Gene Accession Number:
NM_139272.2 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2
