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Gabra5 - Model 6509 - PM

Point Mutation Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

B6;129S6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 6509
    C57BL/6-Gabra5tm1.1(H105R)Twr
  • The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
  • The H105R point mutation was introduced into Gabra5 exon 4.
  • The neomycin resistance cassette, which is flanked by loxP sites, was introduced into intron 3.
  • Chimeric mice were produced and germ line transmission achieved.
  • The neomycin resistance gene has been removed in the constitutive PM mice by crossing to Cre-Deleter mice.
  • The H105R point mutation in exon 4 abolishes benzodiazepine binding of the Gabra5 H105R receptor, but keeps the Gabra5 function otherwise normal.
  • Gabra5 H105R constitutive PM mice are in a mixed C57BL/6-129SvEv genetic background.
  • Unpublished, similar to Gabra1 H101R mice. See R.M. McKernan et al, Nat Neurosci. 2000 Jun;3(6):587-92, for further details.

Gene Family:

Receptor

Genetic Background:

B6:129S6

Gene Symbol:

Gabra5

Gene Accession Number:

NM_176942

Allele Type:

PM

Source:

Merck Research Laboratories

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

gamma-aminobutyric acid (GABA) A receptor, subunit alpha 5

Unpublished, similar to Gabra1H101R mice. See R.M. McKernan et al, Nat Neurosci. 2000 Jun;3(6):587-92, for further details.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

For-Profit Pricing:

Price per Line ($)
$30,000

Non-Profit Pricing:

Price per Line ($)
$5,500

For-Profit Pricing:

Price per Line (€)
€27,300

Non-Profit Pricing:

Price per Line (€)
€5,005