The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
Gabra1 exon 4 was disrupted by the neomycin resistance cassette, which is flanked by loxP sites.
Chimeric mice were produced and germ line transmission achieved.
The neomycin resistance gene has not been deleted in the constitutive KO mice.
Disruption of exon 4 should result in loss of function of the Gabra1 gene by deleting functional domains of the protein.
Gabra1 constitutive KO mice are in a mixed C57BL/6-129SvEv genetic background.
See C. Sur et al, The Journal of Neuroscience 21, 3409-3418, 2001, for further details.
Gene Family: Receptor
Genetic Background: C57BL/6;129SvEv
Gene Symbol: Gabra1
Gene Accession Number: NM_010250
Allele Type: KO
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1