The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 6 to 11 have been flanked by loxP sites (size of loxP-flanked region: 4.0 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 6 to 11 should result in the loss of function of the Fgfr4 gene by deleting half of the second Ig-like C2-type domain, the complete third Ig-like C2-type domain and the transmembrane domain, and by generating a frameshift from exon 5 to exons 12 and 13 (premature Stop codon in exon 12).
Datamining and Design performed in 2010.
Gene Family: Receptor
Genetic Background: C57BL/6
Gene Symbol: Fgfr4
Gene Accession Number: NM_008011.2
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: fibroblast growth factor receptor 4