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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 2 contains the translation initiation codon.
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Exons 6 to 11 have been flanked by loxP sites (size of loxP-flanked region: 4.0 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 6 to 11 should result in the loss of function of the Fgfr4 gene by deleting half of the second Ig-like C2-type domain, the complete third Ig-like C2-type domain and the transmembrane domain, and by generating a frameshift from exon 5 to exons 12 and 13 (premature Stop codon in exon 12).
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Datamining and Design performed in 2010.
Gene Family:
Receptor Genetic Background:
C57BL/6 Gene Symbol:
Fgfr4 Gene Accession Number:
NM_008011.2 Allele Type:
cKO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
fibroblast growth factor receptor 4
