The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Fbxo32 exon 1 contains the translation initiation codon.
Exon 7 has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
Deletion of exon 7 should result in loss of function of the Fbxo32 gene by deleting the essential Fbxo domain.
The remaining recombination sites are located in intron regions of the genome.
Data mining and Design performed in 2006.
Gene Family: Inhibitor
Genetic Background: C57BL/6
Gene Symbol: Fbxo32
Gene Accession Number: NM_026346
Allele Type: cKO
Source: Mouse Clinical Institute (MCI)
Availability: Cryopreserved at Taconic
Official Gene Name: F-box protein 32