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The targeting vector has been transfected into the TaconicArtemis C57BL/6NTac ES cell line.
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The following elements have been inserted into the ROSA26 locus:
- A CAG promoter cassette (containing a cytomegalovirus early enhancer element, a chicken beta-actin promoter and an adenovirus intron).
- The Fads3 open reading frame (based on NCBI transcript NM_021890_3).
- A synthetic polyadenylation signal.
- A Neomycin resistance (NeoR) cassette containing a polyadenylation signal and lacking a translation initiation codon.
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The constitutive TTG line has been generated by crossing chimeras to C57BL/6NTac animals.
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The FADS3 protein is expressed under the control of the CAG promoter.
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The presence of the NeoR cassette terminates the transcription from the ROSA26 promoter.
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Datamining and Design performed in 2010.
Gene Family:
Desaturase Genetic Background:
C57BL/6 Gene Symbol:
Fads3 Gene Accession Number:
NM_021890.3 Allele Type:
TTG Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
fatty acid desaturase 3