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Fabp4&5 - Model 11165 - dKO

Constitutive Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 11165
    C57BL/6-Fabp5tm1.2 MrlFabp4tm2.2Mrl
Note: This is a double KO line: Line 11165 contains both the Fabp5 KO and Fabp4 KO.

  • Fabp5: The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic’s C57BL/6N Tac ES cell line.
  • Fabp5 exon 1 contains the translation initiation codon.
  • Fabp5 exons 1 to 4 have been replaced by the FRT site flanked neomycin resistance gene cassette.
  • The neomycin resistance gene cassette has been removed from the Fabp5 targeted allele by transient transfection of Flp recombinase into targeted ES cell clones.
  • The constitutive Fabp5 KO line has been derived using C57BL/6NTac animals.
  • Deletion of exons 1 to 4 should result in loss of function of the Fabp5 gene by removing the entire coding sequence.
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • Datamining and Design performed in 2008.
  • Fabp4: The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic's C57BL/6NTac ES cell line.
  • Fabp4 exon 1 contains the translation initiation codon.
  • Fabp4 exons 1 to 4 have been replaced by the FRT site flanked neomycin resistance gene cassette.
  • The neomycin resistance gene cassette has been removed from the Fabp4 targeted allele by transient transfection of Flp recombinase into targeted ES cell clones.
  • The constitutive Fabp4 KO line has been derived using C57BL/6NTac animals.
  • Deletion of exons 1 to 4 should result in loss of function of the Fabp4 gene by removing the entire coding sequence.
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • Datamining and Design performed in 2008.

Gene Family:

Enzyme activator

Genetic Background:

C57BL/6

Gene Symbol:

Fabp4, Fabp5

Gene Accession Number:

NM_024406, NM_010634

Allele Type:

dKO

Source:

Xenogen

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

fatty acid binding protein 4, adipocyte, fatty acid binding protein 5, epidermal

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
11165re-allele.png
Fabp5

10896note-allele.png
Fabp4
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.
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