Fabp4&5 - Model 11165 - dKO

Note: This is a double KO line: Line 11165 contains both the Fabp5 KO.

• The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic’s C57BL/6N Tac ES cell line.
• Fabp5 exon 1 contains the translation initiation codon.
• Exons 1 to 4 have been replaced by the FRT site flanked neomycin resistance gene cassette.
• The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
• The constitutive KO line has been derived using C57BL/6NTac animals.
• Deletion of exons 1 to 4 should result in loss of function of the Fabp5 gene by removing the entire coding sequence.
• The remaining recombination sites are located in non-conserved regions of the genome.
• Datamining and Design performed in 2008.

Taconic continues to strive to gain full access to all models for both non-profit and for-profit organizations. However, at this time some models are currently not available to commercial institutions. Please be sure to check back often as more models are made available on a routine basis.

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GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

Nonprofit users: Nonprofit users must provide a scientific workplan and execute an MTA prior to accessing a GEM Collection Model.

Nonprofit order process is as follows:

1. Nonprofit Investigator visits the Taconic website and identifies GEM of interest.
2. Nonprofit Investigator inquires on Taconic website.
3. Taconic will answer questions about the GEM lines and the process.
4. Taconic sends request for MTA.
5. Nonprofit Investigator provides scientific workplan answering 4 basic questions.
6. MTA is issued for nonprofit Investigator/institution review and signature.
7. MTA is executed by all parties and release of model is approved.
8. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery and shipment costs.

Taconic begins cryorecovery upon receipt of PO and delivers model to requestor. It is the requestor's obligation to determine that the GEM meets their research needs.

Access to this model for nonprofit customers requires approval of a third-party Material Transfer Agreement (MTA). The terms of this MTA are non-negotiable; an example agreement is available so that you may preview the terms. MTA approval may require 4-6 months due to circumstances outside our control. Once approved and the purchase confirmed, order fulfillment will require an additional 14-16 weeks. If you wish to continue your interest in this model, please complete the GEM Collection Inquiry Form. In addition, Taconic also offers complete model generation services that may allow for a reduced timeline to delivery of a comparable model. If you are interested in learning more, please request a GEMs Design Consultation.