A plasmid containing the CMV enhancer-chicken β-actin (pCAG) promoter, rabbit b-globin intron and the human ENPP1 cDNA has been engineered.
The plasmid construct was microinjected in C57BL6 mice by pronuclear injection.
Putative transgenic founders were identified by genotyping and bred for germ line transmission with C57BL6 mice.
Heterozygous transgenic mice were identified by genotyping.
Line 10397, C57BL/6-Tg(ENPP1)M119 Mrl, has an overexpression level of the transgene in the brain, muscle and white adipose tissue compared to C57BL6 wild type organs.
Data mining and Design performed in 2007.
Gene Family: Phosphodiesterase
Genetic Background: C57BL/6
Gene Symbol: Enpp1
Gene Accession Number: NM_006208
Allele Type: hTg
Source: Mouse Clinical Institute (MCI)
Availability: Cryopreserved at Taconic
Official Gene Name: ectonucleotide pyrophosphatase/phosphodiesterase 1