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The targeting vector has been generated using C57BL/6J DNA and transfected into the TaconicArtemis C57BL/6NTac ES cell line.
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Dkk1 exon 1 contains the translation initiation codon.
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Exon 2 has been flanked by loxP sites (size of loxP-flanked region: 0.6 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
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Deletion of exon 2 should result in loss of function of the Dkk1 gene by deleting exon 2 and by generating a frameshift from exon 1 to exons 3 and 4.
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The corresponding conditional KO allele is available as line 6092.
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Datamining and Design performed in 2004.
Gene Family:
Receptor ligand Genetic Background:
C57BL/6 Gene Symbol:
Dkk1 Gene Accession Number:
NM_010051 Allele Type:
KO Source:
Merck Research Laboratories Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
dickkopf WNT signaling pathway inhibitor 1
