The targeting vector has been generated using C57BL/6J DNA and transfected into the TaconicArtemis C57BL/6NTac ES cell line.
Dkk1 exon 1 contains the translation initiation codon.
Exon 2 has been flanked by loxP sites (size of loxP-flanked region: 0.6 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exon 2 should result in loss of function of the Dkk1 gene by deleting exon 2 and by generating a frameshift from exon 1 to exons 3 and 4.
The corresponding conditional KO allele is available as line 6092.
Datamining and Design performed in 2004.
Gene Family: Receptor ligand
Genetic Background: C57BL/6
Gene Symbol: Dkk1
Gene Accession Number: NM_010051
Allele Type: KO
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: dickkopf WNT signaling pathway inhibitor 1