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The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
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Disc1 exon 1 contains the translation initiation codon.
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Exon 4 has been flanked by loxP sites.
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 4 should result in loss of function of the Disc1 gene by generating a frameshift (premature stop codon in exon 5). However, not all Disc1 isoforms may be knocked out by this approach.
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The remaining recombination sites are located in intron regions of the genome.
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The corresponding constitutive KO allele is available as line 8349.
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Datamining and Design performed in 2005.
Gene Family:
Disrupted in schizophrenia Genetic Background:
C57BL/6 Gene Symbol:
Disc1 Gene Accession Number:
NM_174853 Allele Type:
cKO Source:
Institut Clinique de la Souris (ICS), Illkirch, France Availability:
Cryopreserved at Taconic
Species:
Mouse
