The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exon 2 has been flanked by loxP sites (size of loxP-flanked region: 1.4 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exon 2 should result in loss of function of the Dhrs4 gene by deleting part of the catalytic domain and by generating a frameshift from exon 1 to exons 3 and 4.
According to the NCBI and Ensembl databases, the BC036313 gene (Entrez ID: 268747) or Lrrc16b gene (ENSMUSG00000022211) is located approx. 10 kb downstream of Dhrs4 exon 2 in a tail-to-head orientation.
Datamining and Design performed in 2009.
Gene Family: Dehydrogenase
Genetic Background: C57BL/6
Gene Symbol: Dhrs4 - Model 10064 - KO
Gene Accession Number: NM_001037938.1
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: dehydrogenase/reductase (SDR family) member 4