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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exon 2 has been flanked by loxP sites (size of loxP-flanked region: 1.4 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exon 2 should result in loss of function of the Dhrs4 gene by deleting part of the catalytic domain and by generating a frameshift from exon 1 to exons 3 and 4.
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According to the NCBI and Ensembl databases, the BC036313 gene (Entrez ID: 268747) or Lrrc16b gene (ENSMUSG00000022211) is located approx. 10 kb downstream of Dhrs4 exon 2 in a tail-to-head orientation.
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Datamining and Design performed in 2009.
Gene Family:
Dehydrogenase Genetic Background:
C57BL/6 Gene Symbol:
Dhrs4 - Model 10064 - KO Gene Accession Number:
NM_001037938.1 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
dehydrogenase/reductase (SDR family) member 4
