Cyp8b1 - Model 11784 - KO

• The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6N Tac ES cell line.
• Cyp8b1 exon 1 contains the complete open reading frame (ORF).
• Exon 1 and 1.9 kb of sequence upstream of exon 1 (promoter region) have been flanked by loxP sites (size of loxP-flanked region:4.1 kb).
• The constitutive KO allele has been obtained after Cre-mediated removal of the exon 1 and the selection marker by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
• The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
• Deletion of exon 1 and the proximal promoter should result in loss of function of the Cyp8b1 gene by removing the entire gene.
• The remaining recombination sites are located in non-conserved regions of the genome.
• The corresponding conditional KO allele is available as line 12015.
• Datamining and Design performed in 2011.

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GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.