The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 3 to 5 have been flanked by loxP sites (size of loxP-flanked region: 1.6 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of the exons 3 to 5 should result in the loss of function of the Cyp11b2 gene by deleting part of the catalytic domain and by generating a frameshift from exon 2 to exon 6 (premature Stop codon in exon 6).
According to NCBI and Ensembl databases, the Cyp11b1 gene (Entrez ID:110115 ; ENSMUSG00000075604) is located approx. 11 kb downstream of Cyp11b2 exon 5, in a tail-to-head orientation.
The corresponding constitutive KO allele is available as line 11430.
Datamining and Design performed in 2010.
Gene Family: Synthase
Genetic Background: C57BL/6
Gene Symbol: Cyp11b2
Gene Accession Number: NM_007806
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: cytochrome P450, family 11, subfamily b, polypeptide 2