NEW MODEL: Jh mouse on B6 background Learn More

Cyp11b2 - Model 11298 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 11298
    C57BL/6-Cyp11b2tm1.1 mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 1 contains the translation initiation codon.
  • Exons 3 to 5 have been flanked by loxP sites (size of loxP-flanked region: 1.6 kb).
  • The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of the exons 3 to 5 should result in the loss of function of the Cyp11b2 gene by deleting part of the catalytic domain and by generating a frameshift from exon 2 to exon 6 (premature Stop codon in exon 6).
  • According to NCBI and Ensembl databases, the Cyp11b1 gene (Entrez ID:110115 ; ENSMUSG00000075604) is located approx. 11 kb downstream of Cyp11b2 exon 5, in a tail-to-head orientation.
  • The corresponding constitutive KO allele is available as line 11430.
  • Datamining and Design performed in 2010.

Gene Family:

Synthase

Genetic Background:

C57BL/6

Gene Symbol:

Cyp11b2

Gene Accession Number:

NM_009991

Allele Type:

cKO

Source:

TaconicArtemis

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

cytochrome P450, family 11, subfamily b, polypeptide 2

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005