- The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
- Cth exon 1 contains the translation initiation codon.
- Exon 6 has been flanked by loxP sites.
- The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
- The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
- Deletion of exon 6 should result in loss of function of the Cth gene by generating a frameshift (premature Stop codon in exon 7).
- The remaining recombination sites are located in intron regions of the genome.
- Datamining and Design performed in 2008.
Gene Family:
Lyase Genetic Background:
C57BL/6 Gene Symbol:
Cth Gene Accession Number:
NM_145953 Allele Type:
KO Source:
Institut Clinique de la Souris (ICS), Illkirch, France Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
cystathionase (cystathionine gamma-lyase)