Cth - Model 9514 - KO

• The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
• Cth exon 1 contains the translation initiation codon.
• Exon 6 has been flanked by loxP sites.
• The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
• The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
• Deletion of exon 6 should result in loss of function of the Cth gene by generating a frameshift (premature Stop codon in exon 7).
• The remaining recombination sites are located in intron regions of the genome.
• Datamining and Design performed in 2008.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.

GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

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