The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic's C57BL/6N Tac ES cell line.
Clec5a exons 1 to 6 have been replaced by the FRT site flanked neomycin resistance gene cassette.
The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
The constitutive KO line has been derived using C57BL/6NTac animals.
Deletion of exons 1 to 6 should result in loss of function of the Clec5a gene.
The remaining recombination sites are located in non-conserved regions of the genome.
Datamining and Design performed in 2004.
Gene Family: Lectin
Genetic Background: C57BL/6
Gene Symbol: Clec5a (Mdl1)
Gene Accession Number: NM_021364
Allele Type: KO
Source: DNAX 3
Availability: Cryopreserved at Taconic
Official Gene Name: C-type lectin domain family 5, member a