The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Chia exon 1 contains the translation initiation codon.
Exon 6 has been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exon 6 should result in loss of function of the Chia gene by deleting the chitinases domain.
The remaining recombination sites are located in intron regions of the genome.
Data mining and Design performed in 2005.
Gene Family: Chitinase
Genetic Background: C57BL/6
Gene Symbol: Chia
Gene Accession Number: NM_023186
Allele Type: KO
Source: Mouse Clinical Institute (MCI)
Availability: Cryopreserved at Taconic
Official Gene Name: chitinase, acidic