The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 3-6 have been flanked by loxP sites (size of loxP-flanked region: 2.1 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 3 to 6 should result in loss of function of the Celsr2 gene by deleting the second EGF-like and the first laminin-G-like domains and by generating a frameshift from exon 2 to exon 7 (premature Stop codon in exon 7).
The remaining recombination sites are located in non-conserved regions of the genome.
The corresponding constitutive KO allele is available as line 10719.
Datamining and Design performed in 2009.
Gene Family: G-protein coupled receptor
Genetic Background: C57BL/6
Gene Symbol: Celsr2
Gene Accession Number: NM_001004177.2
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)