The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exon 4 has been flanked by loxP sites (size of loxP-flanked region: 1.5 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exon 4 should result in loss of function of the Car1 gene by deleting part of the catalytic domain (including residues H95 and H97 described as Zn+-binding sites (SwissProt #P13634)) and by generating a frameshift from exon 3 to exons 5-8 (premature Stop codon in exon 5).
Datamining and Design performed in 2009.
Gene Family: Dehydratase
Genetic Background: C57BL/6
Gene Symbol: Car1
Gene Accession Number: NM_009799.3
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: carbonic anhydrase 1