The targeting vector has been generated using 129SvEv DNA and transfected into 129SvEv ES cell line.
Bud31 (Edg2) coding exon was disrupted by the neomycin resistance cassette, which is flanked by loxP sites.
Chimeric mice were produced and germ line transmission achieved.
The neomycin resistance gene has not been deleted in the constitutive KO mice.
Disruption of the coding sequence should result in loss of function of the Bud31 (Edg2) gene by deleting functional domains of the protein.
Bud31 (Edg2) constitutive KO mice are in a mixed C57BL/6-129SvEv genetic background.
Gene Family: Regulatory factor
Genetic Background: B6:129S6
Gene Symbol: Bud31 (Edg2)
Gene Accession Number: NM_001008705
Allele Type: KO
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: BUD31 homolog (yeast)