Brd4 - Model 13231 - cKO

• The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
• Exon 3 contains the translation initiation codon.
• Exons 6 and 7 have been flanked by loxP sites (size of loxP-flanked region is 1.8 kb).
• The positive selection marker (Puromycin resistance - PuroR) has been flanked by FRT sites and inserted into intron 5.
• The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
• The constitutive KO allele can be generated by Cre-mediated recombination.
• Deletion of exons 6 and 7 should result in the loss of function of the Brd4 gene by generating a frameshift from exon 5 to exons 8-11 (premature Stop codon in exon 8).
• The remaining recombination sites will be located in non-conserved regions of the genome.
• Datamining and Design performed 2013.

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GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

• A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
• Validated genotyping protocols
• Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.

Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.

• GEM Collection Commercial Terms of Sale
• GEM Collection Academic Terms of Sale

The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.