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Brd3 - Model 13333 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 13333
    C57BL/6-Brd3tm1.1Mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 2 contains the translation initiation codon.
  • Exon 5 has been flanked by loxP sites (size of loxP-flanked region: 0.9 kb).
  • The positive selection marker (Puromycin resistance - PuroR) has been flanked by FRT sites and inserted into intron 5.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination.
  • Deletion of exon 5 should result in the loss of function of the Brd3 gene by generating a frameshift from exon 4 to exons 6-11 (premature Stop codon in exon 6).
  • The remaining recombination sites will be located in non-conserved regions of the genome.
  • Datamining and Design performed 2013.

Gene Family:

Transcriptional regulator

Genetic Background:

C57BL/6

Gene Symbol:

Brd3

Gene Accession Number:

ENSMUST00000113941

Allele Type:

cKO

Source:

TaconicArtemis

Species:

Mouse

Official Gene Name:

bromodomain containing 3

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.