The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exon 7 has been flanked by loxP sites (size of loxP-flanked region: 1.0 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exon 7 should result in loss of function of the Atg4b gene by removing part of the Cysteine endopeptidase domain and by generating a frameshift from exon 6 to all downstream exons (premature translation STOP codon will occur in exon 8).
Datamining and Design performed in 2008.
Gene Family: Peptidase
Genetic Background: C57BL/6
Gene Symbol: Atg4b
Gene Accession Number: NM_174874.2
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: autophagy-related 4B (yeast)