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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exon 7 has been flanked by loxP sites (size of loxP-flanked region: 1.0 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exon 7 should result in loss of function of the Atg4b gene by removing part of the Cysteine endopeptidase domain and by generating a frameshift from exon 6 to all downstream exons (premature translation STOP codon will occur in exon 8).
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Datamining and Design performed in 2008.
Gene Family:
Peptidase Genetic Background:
C57BL/6 Gene Symbol:
Atg4b Gene Accession Number:
NM_174874.2 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
autophagy-related 4B (yeast)