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Acss2 - Model 10365 - KO

Constitutive Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 10365
    C57BL/6-Acss2tm1.2 mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 1 contains the translation initiation codon.
  • Exons 3 to 7 have been flanked by loxP sites (size of loxP-flanked region: 2.0 kb).
  • The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
  • The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
  • Deletion of exons 3-7 should result in loss of function of the Acss2 gene by deleting the transmembrane domain and part of the catalytic domain and by generating a frameshift from exon 2 to downstream exons (premature Stop codon in exon 8).
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Acss2 gene:

    • The Ggtl3 gene (Entrez ID: 207182; ENSMUSG00000027603) is located approx. 34 kb upstream of Acss2 exon 3 in a head-to-head orientation.
    • The Gss gene (Entrez ID: 14854; ENSMUSG00000027610) is located approx. 13 kb downstream of Acss2 exon 7 in a tail-to-tail orientation.

  • Datamining and Design performed in 2009.

Gene Family:


Genetic Background:


Gene Symbol:


Gene Accession Number:


Allele Type:





Cryopreserved at Taconic



Official Gene Name:

acyl-CoA synthetase short-chain family member 2

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GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.