The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Acsm3 exon 4 contains the translation initiation codon.
Exon 6 has been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exon 6 should result in loss of function of the Acsm3 gene by generating a frameshift (premature Stop codon in exon 7).
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2007.
Gene Family: Synthetase
Genetic Background: C57BL/6
Gene Symbol: Acsm3
Gene Accession Number: NM_016870
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: acyl-CoA synthetase medium-chain family member 3