- The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
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Acsm1 exon 2 contains the translation initiation codon.
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Exon 4 has been flanked by loxP sites.
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
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Deletion of exon 4 should result in loss of function of the Acsm1 gene by deleting the AMP-dependent synthetase/ligase domain.
- The remaining recombination sites are located in intron regions of the genome.
- The corresponding constitutive KO allele is available as line 14183.
- Data mining and Design performed in 2008.
Gene Family:
Synthetase Genetic Background:
C57BL/6 Gene Symbol:
Acsm1 Gene Accession Number:
NM_054094 Allele Type:
cKO Source:
Institut Clinique de la Souris (ICS), Illkirch, France Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
acyl-CoA synthetase medium-chain family member 1
