- The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Acsm1 exon 2 contains the translation initiation codon.
Exon 4 has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
Deletion of exon 4 should result in loss of function of the Acsm1 gene by deleting the AMP-dependent synthetase/ligase domain.
- The remaining recombination sites are located in intron regions of the genome.
- The corresponding constitutive KO allele is available as line 14183.
- Data mining and Design performed in 2008.
Gene Family: Synthetase
Genetic Background: C57BL/6
Gene Symbol: Acsm1
Gene Accession Number: NM_054094
Allele Type: cKO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: acyl-CoA synthetase medium-chain family member 1