The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Acsm1 exon 2 contains the translation initiation codon.
Exon 4 has been flanked by loxP sites.
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
Deletion of exon 4 should result in loss of function of the Acsm1 gene by deleting the AMP-dependent synthetase/ligase domain.
The remaining recombination sites are located in intron regions of the genome.
The corresponding constitutive KO allele is available as line 14183.
Data mining and Design performed in 2008.
Gene Family: Synthetase
Genetic Background: C57BL/6
Gene Symbol: Acsm1
Gene Accession Number: NM_054094
Allele Type: cKO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: acyl-CoA synthetase medium-chain family member 1