The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 2 to 6 have been flanked by loxP sites (size of loxP-flanked region: 2.6 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 2 to 6 should result in loss of function of the Acsf2 gene by deleting part of the catalytic domain and by generating a frameshift from exon 1 to exons 7 and 8 (premature Stop codon in exon 7).
The remaining recombination sites are located in non-conserved regions of the genome.
According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Acsf2 gene:
- The Chad gene (Entrez ID:12643; ENSMUSG00000039084) is located into Acsf2 intron 10 (182 bp downstream of Acsf2 exon 10) in tail-to-tail orientation.
- The Rsad1 gene (Entrez ID:237926; ENSMUSG00000039096) is located 22 kb downstream of Acsf2 exon 6 in a head-to-tail orientation.
The corresponding constitutive KO allele is available as line 10908.
Datamining and Design performed in 2009.
Gene Family: Synthetase
Genetic Background: C57BL/6
Gene Symbol: Acsf2
Gene Accession Number: NM_153807.2
Allele Type: cKO
Availability: Cryopreserved at Taconic
Official Gene Name: acyl-CoA synthetase family member 2