-
The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
-
Acly exon 2 contains the translation initiation codon.
-
Exons 17-19 have been flanked by loxP sites.
-
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
-
The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
-
Deletion of exons 17-19 should result in loss of function of the Acly gene by abolishing the essential binding domains.
-
The remaining recombination sites are located in intron regions of the genome.
-
The constitutive KO allele is available as line 10412.
-
Data mining and Design performed in 2004.
Gene Family:
Synthetase Genetic Background:
C57BL/6 Gene Symbol:
Acly Gene Accession Number:
NM_134037 Allele Type:
KO Source:
Mouse Clinical Institute (MCI) Availability:
Cryopreserved at Taconic
Species:
Mouse