The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6J ES cell line.
Acer3 (Phca) exon 1 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exon 3 should result in loss of function of the Acer3 (Phca) gene by deleting a transmembrane region.
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2005.
Gene Family: Hydrolase
Genetic Background: C57BL/6
Gene Symbol: Acer3
Gene Accession Number: NM_025408
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: alkaline ceramidase 3