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The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6J ES cell line.
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Acer3 (Phca) exon 1 contains the translation initiation codon.
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Exon 3 has been flanked by loxP sites.
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
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Deletion of exon 3 should result in loss of function of the Acer3 (Phca) gene by deleting a transmembrane region.
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The remaining recombination sites are located in intron regions of the genome.
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Datamining and Design performed in 2005.
Gene Family:
Hydrolase Genetic Background:
C57BL/6 Gene Symbol:
Acer3 Gene Accession Number:
NM_025408 Allele Type:
KO Source:
Institut Clinique de la Souris (ICS), Illkirch, France Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
alkaline ceramidase 3
