Rag2/OT-II (11490)

Constitutive Knockout / Random Transgenic

Rag2/OT-II Constitutive Knockout/Random Transgenic Mouse Model
This model is impacted by a newly discovered spontaneous mutation in the Thyroglobulin (Tg) gene. It will be removed over time through selective breeding. See the Fact Sheet for more information.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 11490-F
    B6.129S6-Rag2tm1Fwa Tg(TcraTcrb)425Cbn
  • 11490-M
    B6.129S6-Rag2tm1Fwa Tg(TcraTcrb)425Cbn
  • Carries a transgene that encodes for a T cell receptor (Valpha2 and Vbeta5) specific for a chicken ovalbumin peptide (323-339) presented by the MHC class II molecule H2-Ab1 (I-Ab)
  • Deficient in the recombination activating gene 2 (Rag2) and therefore does not develop mature T and B cells expressing endogenous T cell receptors
  • Virtually all CD4+ cells express the transgene
  • Useful as a source of homogenous donor CD4+ cells for in vivo adoptive transfer studies to investigate T cell development, memory, activation and tolerance
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Genetic Background:

C57BL/6 Background


The OT-II microinjected model was developed by Dr. Francis Carbone of Monash Medical School and Dr. William R. Heath of the Walter and Eliza Hall Institute. The model was created by microinjecting the rearranged Valpha2 and Vbeta5 T cell receptor into B6 x B6.C-H2bm1 blastocysts. Taconic received stock from Keio University in June 2001, and the mice were rederived by backcrossing to C57BL/6NTac. OT-II transgenic mice were crossed with a homozygous C57BL/6NTac Rag2 targeted mutation model (RAGN12-M), and then intercrossed for homozygosity at the Rag2 allele. The model was rederived by embryo transfer and is maintained by mating of mice that are homozygous for Rag2 disruption and heterozygous for the OT-II transgene to mice that are homozygous for the Rag2 disruption and wild type for OT-II.





Initial Publication:

Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.

Barnden MJ, Allison J, Heath WR, Carbone FR. (1998) Defective TCR expression in transgenic mice constructed using cDNA-based α- and Β-chain genes under the control of heterologous regulatory elements. Immunol Cell Biol 76:34-40.

Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic.
  • The Models will be used for research purposes only.
  • The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.

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