Rag2 - Model 461

Constitutive Knock Out

Rag2 (Model 461) Constitutive Knock Out Mouse Model
EZcohort™ Models
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B6.SJL Background

  • Model #
  • Genotype
  • Nomenclature
  • 461-F
    ko/ko
    B6.SJL(129S6)-Ptprca/BoyCrTac-Rag2tm1Fwa N10
  • 461-M
    ko/ko
    B6.SJL(129S6)-Ptprca/BoyCrTac-Rag2tm1Fwa N10
  • Contains a disruption of the recombination activating gene 2 (Rag2)
  • Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes.
  • Useful for vaccine development, transplantation studies and hematopoiesis research.
  • The Ptprca marker on hematopoietic cells of this congenic strain allows for their identification in immunological adoptive transfer experiments.
  • Similar to C57BL/6 with the H2b haplotype but carries the Ptprca and Pep3b genes from the SJL strain.

Genetic Background:

B6.SJL Background

Origin:

The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts. Ellis Reinherz of the Dana Farber Cancer Institute received Rag2 mice from the Alt lab on a mixed background. The mice were then backcrossed by S. Koyasu for 10 generations (N10) to B6.SJL-Ptprcα (from Cancer Research). Taconic received stock in 1996. The mice were derived by embryo transfer, and the colony was maintained by mating homozygous mice.


Color:

Black

Species:

Mouse

Initial Publication:

Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.



Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.