Rag1 Knockout/Transgenic OT-II T Cell Receptor

Constitutive Knockout

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The NIAID Exchange Program has closed and live production of this model has ceased. Please consider the Rag2/OT-II which is considered an equivalent substitute.

C57BL/6 Background

Model #
Genotype
Nomenclature

4234-F

B6.129S7-Rag1^tm1Mom Tg(TcraTcrb)425Cbn
4234-M

B6.129S7-Rag1^tm1Mom Tg(TcraTcrb)425Cbn

Model Description

The Rag1 Knockout/Transgenic OT-II T Cell Receptor line is homozygous for a transgene that encodes a T cell receptor specific for chicken ovalbumin (amino acids 323-339), presented by the MHC class II molecule I-Ab. The OT-II transgene is autosomal in contrast to a second subline in which the transgene is located on the Y chromosome. It is also deficient in the Rag1 gene (recombinase reactivating gene 1) and therefore does not develop endogenous mature T or B cells. Virtually all peripheral CD4+ cells express the two transgenes. These mice are useful as a source of homogeneous donor CD4+ T cells for in vivo adoptive transfer studies to investigate T cell development, activation, memory, and tolerance. They can be used in conjunction with the Rag1 Knockout/Transgenic OT-I T Cell Receptor CD8+ T cells, specific for chicken ovalbumin 257-264 presented by the MHC class I molecule H2-K^b, as a pair of helper and CTL T cells reactive against the same antigen.

Genetic Background:

C57BL/6 Background

Origin:

The Transgenic OT-II T Cell Receptor mouse was developed by Dr. Francis Carbone of Monash Medical School and Dr. William R. Heath of the Walter and Eliza Hall Institute. The model was created by microinjecting the rearranged Vα2 and Vβ5 T cell receptor into B6 x B6.C-H2bm1 blastocysts. Founders were subsequently bred to B6 mice. The line was later transferred to the NIAID repository at Taconic from Eric Butz of the Immunex Corporation. The transgenic mice were crossed twice to a homozygous Rag1 knockout mouse on a B6 background (Mombaerts P, Cell 1992;68:869-877, generated through gene targeting in 129S7-derived ES cells) and Rag1-/- offspring were selected. These mice were then intracrossed to produce the line homozygous for the TCR transgene. TCR positive mice homozygous for the transgene were identified by test mating the mice to wild type partners and selecting animals that only gave rise to transgene positive offspring.

Availability:

This model is unavailable.

Color:

Black

Species:

Mouse

Initial Publication:

Barnden MJ, Allison J, Heath WR ,Carbone FR; Defective TCR expression in transgenic mice constructed using cDNA-based α- and β- chain genes under the control of heterologous regulatory elements. Immunol Cell Biol February 1998 76(1) pp.34-40.
Li M, Davey GM, Sutherland RM, Kurts C, Lew AM, Hirst C, Carbone FR, Heath WR.; Cell-associated ovalbumin is cross-presented much more efficiently than soluble ovalbumin in vivo. J Immunol 2001 166 pp.6099-6103.
Mombaerts P, Iacomini J, Johnson RS, Herrup I, Tonegawa S, Papaioannou VE. 1992. RAG-1 deficient mice have no mature B and T lymphocytes. Cell 68: 869-877.