Constitutive Knockout

Pfp/Rag2 Constitutive Knockout Mouse Model
EZcohort® Models
The easiest way to acquire and breed cryopreserved GEMs

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 1177-F
    B6.129S6-Rag2tm1FwaPrf1tm1Clrk N12
  • 1177-M
    B6.129S6-Rag2tm1FwaPrf1tm1Clrk N12
  • This double targeted mutation contains a disruption of both Rag2 and Pfp genes
  • Exhibits a severe depletion of NK cell function through the disruption of the Pfp gene and lacks mature T or B lymphocytes through disruption of the Rag2 gene
  • Used to study overall regulation of the immune system or specific areas such as NK or CTL activity, immune suppression and transplantation
  • Carries the H2b haplotype

Genetic Background:

C57BL/6 Background


The Pfp/Rag2 mouse was developed by crossbreeding the Pfp targeted mutation mouse (model PFPN12) and the Rag2 targeted mutation mouse (model RAGN12) at Taconic and breeding to homozygosity for both genes. This model was backcrossed twelve generations (N12) to C57BL/6NTac. The colony was maintained through homozygous matings.


Wild type for Nnt mutation





Initial Publication:

Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.

Walsh CM, Matloubian M, Liu C, Ueda R, Kurahara CG, Christensen JL, Huang MTF, Young JD, Ahmed R, and Clark WR. (1994) Immune function in mice lacking the perforin gene. Proc Natl Acad Sci USA, 91(23): 10854-10858.

Conditions of Use for EZcohort® Models
EZcohort® models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort® models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort® models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort® models and biological materials derived from them remains with Taconic.
  • EZcohort® models will be used for research purposes only.
  • EZcohort® models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort® models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort® models outside Taconicis permitted for a term of three years post-purchase of the EZcohort® models and is subject to annual confirmation of these EZcohort® Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort® Conditions of Use and payment of a fee.