HLA-B7 (B6;C)

Constitutive Knockout / Random Transgenic

HLA-B7 (B6;C)
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C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 8908-F
    ko/wt;tg/wt;tg/wt
    B6;C B2mtm1Unc Tg(HLA-B*0702/H2-Kb)B7.xx Tg(B2M)55Hpl
  • 8908-M
    ko/wt;tg/wt;tg/wt
    B6;C B2mtm1Unc Tg(HLA-B*0702/H2-Kb)B7.xx Tg(B2M)55Hpl

  • Carries a transgene consisting of fragments of the human HLA-B*0702 gene and mouse H2-Kb gene which encodes a chimeric class I molecule consisting of the human HLA-B7 leader, α1 and α2 domains ligated to the murine α3, transmembrane and cytoplasmic H2-Kb domains.
  • Expresses the chimeric HLA-B7 class I molecule on the surface of T and B cells.
  • Represents the human HLA-B7 supertype.
  • Useful for infectious disease research, vaccine development and testing, safety and immunogenicity testing as well as research directed towards oncology, autoimmune disorders, and syngeneic tumor studies utilizing C57BL/6-derived tumors.
  • Permits identification of epitopes restricted to the HLA-B7 supertype.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Genetic Background:

C57BL/6

Origin:

The HLA-B7 model was generated by Pharmexa-Epimmune. The model was created by microinjecting a chimeric transgene combining a fragment of the cDNA clone of the human HLA-B*0702 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-Kb gene containing the α3, transmembrane and cytoplasmic domains, all under the control of the murine H2-Kb promoter. This transgene was microinjected into CxB6 zygotes. The resultant mice from Founder Line B7.xx were on backcrossed four generations to C57BL/6J. They were then crossed onto murine B2m knockout/human B2m microinjected line from Chella David at Mayo. This crossbred line was backcrossed two additional generations to C57BL/6J and then bred to homozygosity for all three genes. Taconic received stock from Pharmexa-Epimmune in 2008. The line was embryo transfer derived and backcrossed to C57BL/6NTac for a total of 6 generations. SNP testing showed that the line was not congenic, and so additional backcrosses were initiated, which have not completed yet. The line was then intracrossed to homozygosity for all three genes. In the production colony, wild type C57BL/6NTac animals are bred to triple homozygous animals on the mixed B6 background to produce hemizygous pups. The saleable colony is current at NE6 on the C57BL/6NTac background. In addition, another production is breeding BALB/cAnNTac females with homozygous males on the mixed C57BL/6NTac background to produce hemizygous CB6F1 pups with are sold under model #9661.


Availability:

HLA-B7 mice are produced upon order. Available for order now.

Color:

Black

Species:

Mouse

Initial Publication:

  • Alexander J, Oseroff C, Sidney J, Sette A. (2003) Derivation of HLA-B*0702 transgenic mice: functional CTL repertoire and recognition of human B*0702-restricted CTL epitopes. Human Immunology 64:211-223.
  • Koller BH, Smithies O. (1989) Inactivating the beta 2-microglobulin locus in mouse embryonic stem cells by homologous recombination. Proc Natl Acad Sci USA 86:8932-8935.
  • Krimpenfort P, Rudenko G, Hochstenbach F, Guessow D, Berns A, Ploegh H. (1987) Crosses of two independently derived transgenic mice demonstrate functional complementation of the genes encoding heavy (HLA-B27) and light (beta 2-microglobulin) chains of HLA class I antigens. EMBO J, 1987, 6(6):1673-1676.


Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:
  • Title to these Models and biological materials derived from them remains with Taconic.
  • The Models will be used for research purposes only.
  • The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.

HLA expression on spleen cells in HLA-B7 mice on two genetic backgrounds

Figure 1: Expression of HLA-B7 in model #s 8908 and 9661 was measured on spleen cells by flow cytometry using a pan-HLA-ABC antibody. Expression levels are somewhat lower in hemizygous mice of model #9661 (on a CB6F1 background) compared to homozygous or hemizygous mice of model #8908 (on a C57BL/6 background), but are sufficient for experimental performance as detailed in many published references. Specific functional relevance of expression differences between models is unclear, and the choice of model for a particular experiment should include not just expression levels but also the role of Th1 vs Th2 immune response, whether a congenic background is required for use in syngeneic engraftment or adoptive cell transfer experiments and other factors.
Figure 1: Expression of HLA-B7 in model #s 8908 and 9661 was measured on spleen cells by flow cytometry using a pan-HLA-ABC antibody. Expression levels are somewhat lower in hemizygous mice of model #9661 (on a CB6F1 background) compared to homozygous or hemizygous mice of model #8908 (on a C57BL/6 background), but are sufficient for experimental performance as detailed in many published references. Specific functional relevance of expression differences between models is unclear, and the choice of model for a particular experiment should include not just expression levels but also the role of Th1 vs Th2 immune response, whether a congenic background is required for use in syngeneic engraftment or adoptive cell transfer experiments and other factors.

HLA expression histogram for spleen cells in HLA-B7 mice on two genetic backgrounds

Figure 2: Histograms for HLA-B7 expression on spleen cells in representative animals, as measured using a pan-HLA-ABC antibody. Grey = wild type C57BL/6 control. Blue = three model #8908 hemizygous female mice (C57BL/6 background). Red = three model #9661 hemizygous female mice (CB6F1 background).
Figure 2: Histograms for HLA-B7 expression on spleen cells in representative animals, as measured using a pan-HLA-ABC antibody. Grey = wild type C57BL/6 control. Blue = three model #8908 hemizygous female mice (C57BL/6 background). Red = three model #9661 hemizygous female mice (CB6F1 background).
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