HLA-B7

Genetic Background:

Origin:

The HLA-B7 model was generated by Pharmexa-Epimmune. The model was created by microinjecting a chimeric transgene combining a fragment of the cDNA clone of the human HLA-B*0702 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-K^b gene containing the α3, transmembrane and cytoplasmic domains, all under the control of the murine H2-K^b promoter. This transgene was microinjected into CxB6 zygotes. The resultant mice from Founder Line B7.xx were on backcrossed four generations to C57BL/6J. They were then crossed onto murine B2m knockout/human B2m microinjected line from Chella David at Mayo. This crossbred line was backcrossed two additional generations to C57BL/6J and then bred to homozygosity for all three genes. Taconic received stock from Pharmexa-Epimmune in 2008. The line was embryo transfer derived. SNP testing showed that the line was not congenic, and so additional backcrosses were initiated. The line was then intracrossed to homozygosity for all three genes. In the production colony, wild type BALB/cAnNTac females are bred to triple homozygous males on the B6 background to produce CB6F1 pups.

Availability:

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Species:

Initial Publication:

Alexander J, Oseroff C, Sidney J, Sette A. (2003) Derivation of HLA-B*0702 transgenic mice: functional CTL repertoire and recognition of human B*0702-restricted CTL epitopes. Human Immunology 64:211-223.

Koller BH, Smithies O. (1989) Inactivating the beta 2-microglobulin locus in mouse embryonic stem cells by homologous recombination. Proc Natl Acad Sci USA 86:8932-8935.

Krimpenfort P, Rudenko G, Hochstenbach F, Guessow D, Berns A, Ploegh H. (1987) Crosses of two independently derived transgenic mice demonstrate functional complementation of the genes encoding heavy (HLA-B27) and light (beta 2-microglobulin) chains of HLA class I antigens. EMBO J, 1987, 6(6):1673-1676.

Animal Diet:

• Title to these Models and biological materials derived from them remains with Taconic Biosciences, Inc.
• The Models will be used for research purposes only.
• The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless a breeding or cross-breeding agreement has been legally executed. Non-profit purchasers, of a breeding or cross-breeding agreement, may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless the breeding or cross-breeding agreement is purchased at the for-profit price. Please contact Taconic Customer Service for details.
• The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.