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Carries a transgene consisting of fragments of the human HLA-A*0201 gene and mouse H2-Kb gene which encodes a chimeric class I molecule consisting of the human HLA-A2.1 leader, α1 and α2 domains ligated to the murine α3, transmembrane and cytoplasmic H2-Kb domains
Expresses the chimeric HLA-A2.1 class I molecule on the surface of T and B cells
Represents the human HLA-A2 supertype
Useful for infectious disease research, vaccine development and testing, safety and immunogenicity testing as well as research directed towards oncology and autoimmune disorders
Permits identification of epitopes restricted to the HLA-A2 supertype
Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background: CB6F1 Background
Origin: The HLA-A2.1 model was generated by Pharmexa-Epimmune. The model was created by microinjecting a chimeric transgene combining a fragment of the genomic clone of the human HLA-A*0201 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-Kb gene containing the α3, transmembrane and cytoplasmic domains. This transgene was microinjected into C57BL/6J zygotes. The resultant mice from Founder Line A*0201 were on an inbred C57BL/6J background. Taconic received stock from Pharmexa-Epimmune in 2008. The line was embryo transfer derived, and the colony is maintained through breeding of homozygous males and females. In the production colony, wild type BALB/cAnNTac females are bred to homozygous males on the C57BL/6J background to produce CB6F1 pups.
Color: Black Agouti
Vitiello A, Marchesini D, Furze J, Sherman LA, Chesnut RW. (1991) Analysis of the HLA-restricted influenza-specific cytotoxic T lymphocyte response in transgenic mice carrying a chimeric human-mouse class I major histocompatibility complex. J Exp Med 173(4):1007-15.
* This reference describes the transgene incorporated into line 9659. Pharmexa-Epimmune remade the line later directly onto B6/J zygotes. This is the mouse line that Taconic offers as Model # 9659.