- Carries a transgene consisting of fragments of the human HLA-A*1101 gene and mouse H2-Kb gene which encodes a chimeric class I molecule consisting of the human HLA-A11 leader, α1 and α2 domains ligated to the murine α3, transmembrane and cytoplasmic H2-Kb domains.
- Expresses the chimeric HLA-A11 class I molecule on the surface of T and B cells.
- Represents the human HLA-A3 supertype.
- Useful for infectious disease research, vaccine development and testing, safety and immunogenicity testing as well as research directed towards oncology, autoimmune disorders, and syngeneic tumor studies utilizing C57BL/6-derived tumors.
- Permits identification of epitopes restricted to the HLA-A11 supertype.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Genetic Background:
C57BL/6 Origin:
The HLA-A11 model was generated by Pharmexa-Epimmune. The model was created by microinjecting a chimeric transgene combining a fragment of the cDNA clone of the human HLA-A*1101 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-K
b gene containing the α3, transmembrane and cytoplasmic domains. This transgene was microinjected into CxB6 zygotes. The resultant mice from Founder Line A11.01 were backcrossed six generations to C57BL/6J. Taconic received stock from Pharmexa-Epimmune in 2008. The line was embryo transfer derived, backcrossed to C57BL/6NTac for a total of 11 generationsand then intracrossed to homozygosity. In the production colony, wild type C57BL/6NTac animals are bred to homozygous males to produce hemizygous pups on the C57BL/6NTac background. In addition, another production colony is breeding BALB/cAnNTac females with homozygous males on the C57BL/6NTac background to produce hemizygous CB6F1 pups, which are sold under
model #9660.
Color:
Black Species:
Mouse Initial Publication:
Alexander J, Oseroff C, Sidney J, Wentworth P, Keogh E, Hermanson G, Chisari FV, Kubo RT, Grey HM, Sette A. (1997) Derivation of HLA-A11/Kb transgenic mice: functional CTL repertoire and recognition of human A11-restricted CTL epitopes. J Immunol 159(10):4753-61. Erratum in:
J Immunol 1999 162(5):3104.