Generating EAE Mouse Models of Multiple Sclerosis


     
Experimental Autoimmune Encephalomyelitis (EAE) is a widely-accepted model of demyelinating diseases, such as multiple sclerosis (MS). How can you most efficiently generate EAE mouse models of multiple sclerosis and which mouse strains work best for this application?

Although the exact details are not understood, the immune system is involved in MS pathogenesis. Like MS, EAE is characterized by infiltration of immune cells into the central nervous system (CNS) and demyelination. The two most widely-used methods to induce EAE are:
  • Active induction by immunization.
  • Passive induction through adoptive transfer.
Each approach has its own strengths and potential complications, so you should settle on an EAE induction method in the early stages of your study design.

EAE by Active Immunization

Active immunization models are the most popular mouse models of multiple sclerosis. In the active immunization method, the mouse is immunized using neuro antigens, such as myelin antigens or spinal cord homogenate, which causes development of activated myelin-specific T cells, trafficking of immune cells into the CNS, and direct damage and inflammation in the CNS.

General Benefits and Considerations

  • Quick, relatively easy, and inexpensive.
  • Active immunization in Black 6 mice is suitable for compound profiling.
  • Active immunization in SJL mice induces a relapsing/remitting disease phenotype.
  • Antigens for EAE induction include myelin oligodendrocyte glycoprotein (MOG), myelin basic protein (MBP), proteolipid protein (PLP), and spinal cord homogenate.
  • Most protocols call for the use of pertussis toxin (PTX), which increases both the incidence and severity of disease.
  • Disease presents as ascending paralysis and the typical readout is clinical score. Histology may be used to visualize inflammation in the CNS.
    • The Gfap-luc mouse model offers an additional readout: in vivo imaging of bioluminescence as a marker of neuroinflammation. (Note that the Gfap-luc model on the B6 albino x FVB hybrid background must be used in order to maintain susceptibility to EAE.)

Basic Protocol

Chronic EAE Model

Relapsing/Remitting Model

Mice are immunized with peptide on day 0 and given pertussis toxin (PTX) on day 1 and 2. Onset of clinical illness is typically observed on days 9-14, with peak severity at days 12-19. For relapsing remitting models, days 14-21 partial recovery will be measurable with 25-30% of mice relapsing on days 20-29.

Adoptive Transfer EAE Mouse Models

In the adoptive transfer model of EAE, one set of mice is directly immunized to generate myelin-specific T cells which are then cultured and transferred into donor mice.

General Benefits and Considerations

  • Rapid onset and increased disease severity.
  • The adoptive transfer model permits separation of the effector phase from induction.
  • Congenic pairs of donors and recipients can be used to study cell trafficking. Using B6.SJL-Ptprca mice as donors and C57BL/6 mice as recipients, donor T cells can be tracked in vivo in the recipient mice.
  • Study differentiated Th1 or Th17 populations; in vitro culture conditions can be varied to alter the dominant cell type in the transferred cells.
Adoptive Transfer EAE Mouse Models

Adoptive Transfer EAE Mouse Models

Strain Selection for EAE Mouse Models

Black 6 on the J and NTac background exhibit comparable EAE development and clinical scores, with C57BL/6NTac having slightly higher clinical scores in the initial onset and the J having slightly higher relapse scores.

Age and sex matched Black 6 mice used in active immunization study using MOG and CFA

Age and sex matched Black 6 mice used in active immunization study using MOG and CFA. Protocol available from Hooke Labs.
C57BL/6 Mice from different Taconic barriers with the same health status

C57BL/6 Mice from different Taconic barriers with the same health status. There is consistency in data due to harmonization. Data collected by Hooke Labs.
For more information on the Taconic models mentioned and their applications see:
References and Suggested Reading:
1. "Multiple sclerosis FAQs." The National Multiple Sclerosis Society. http://www.nationalmssociety.org/What-is-MS/MS-FAQ-s. Accessed Oct 27, 2016.
2. McCarthy, D. P., Richards, M. H., & Miller, S. D. (2012). Mouse Models of Multiple Sclerosis: Experimental Autoimmune Encephalomyelitis and Theiler's Virus-Induced Demyelinating Disease. Methods in Molecular Biology (Clifton N.J.), 900, 381-401.
3. Muller, D. M., Pender, M. P., & Greer, J. M. (2005). Blood-brain barrier disruption and lesion localisation in experimental autoimmune encephalomyelitis with predominant cerebellar and brainstem involvement. Journal of Neuroimmunology, 160(1), 162-169.
4. Croxford AL, Kurschus FC, Waisman A, (2011) Mouse models for multiple sclerosis: Historical facts and future implications. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1812(2)177-83.
5. Constantinescu, C. S., Farooqi, N., O'Brien, K., & Gran, B. (2011). Experimental autoimmune encephalomyelitis (EAE) as a model for multiple sclerosis (MS). British Journal of Pharmacology, 164(4), 1079-1106.
6. Fletcher, J. M., Lalor, S. J., Sweeney, C. M., Tubridy, N., & Mills, K. H. G. (2010). T cells in multiple sclerosis and experimental autoimmune encephalomyelitis. Clinical and Experimental Immunology, 162(1), 1-11.
7. Mony, J. T., Khorooshi, R., & Owens, T. (2014). MOG extracellular domain (p1-125) triggers elevated frequency of CXCR3+ CD4+ Th1 cells in the CNS of mice and induces greater incidence of severe EAE. Multiple Sclerosis Journal, 1352458514524086.
8. Brambilla, R., Morton, P. D., Ashbaugh, J. J., Karmally, S., Lambertsen, K. L., & Bethea, J. R. (2014). Astrocytes play a key role in EAE pathophysiology by orchestrating in the CNS the inflammatory response of resident and peripheral immune cells and by suppressing remyelination. Glia, 62(3), 452-467.
9. Mony, J. T., Khorooshi, R., & Owens, T. (2014). Chemokine receptor expression by inflammatory T cells in EAE. Chemokines and Chemokine Receptors in Brain Homeostasis, 6.
10. Isaksson, M., Lundgren, B. A., Ahlgren, K. M., Kämpe, O., & Lobell, A. (2012). Conditional DC depletion does not affect priming of encephalitogenic Th cells in EAE. European Journal of Immunology, 42(10), 2555-2563.
11. Burkett, P. R., zu Horste, G. M., & Kuchroo, V. K. (2015). Pouring fuel on the fire: Th17 cells, the environment, and autoimmunity. The Journal of Clinical Investigation, 125(6), 2211-2219.