Metabolic Disease INSIGHTS

In a recent webinar, Dr. Michael Briggs and Mrs. Nikole Siegmund of Woodland Biosciences discussed use of diet-induced obese (DIO) mice for metabolic disease research. The speakers explained how DIO mice can be used in a range of study types, including: Obesity reduction Obesity-related inflammation Diabetes readouts such as glucose Obesity-related cancer Examples of typical studies using real (anonymized) data were shown. Obesity-driven metabolic diseases, including non-alcoholic...

On February 2, 2018, researchers at The Bartholin Institute, Biocenter in Copenhagen, Denmark, published a paper titled Gluten‐free diet during pregnancy alleviates signs of diabetes and celiac disease in NOD mouse offspring in the journal Diabetes Metabolism Research Reviews. NOD mice have been the model of choice for autoimmune Type 1 diabetes research for over 30 years as they are a polygenic model of diabetes that mimic...

The global obesity epidemic has pushed associated liver diseases to the forefront of research, yet no existing animal model of NASH or NAFLD fully recapitulates its clinical presentation. This presents a challenge for effective study design: how do you select the most appropriate and translatable animal model for studying the pathophysiology of NASH? Causes of NASH in Humans Non-alcoholic fatty liver disease (NAFLD) affects 30-40% of US...

There is a growing body of evidence that time-restricted feeding (TRF), for example limiting eating to the active versus inactive phase for a particular species, can impact weight gain and development of obesity. Researchers at the Norwegian University of Science and Technology, the Central Norway Regional Health Authority, and Trondheim University Hospital recently demonstrated that TRF restricts weight gain in juvenile rats. Most previous literature on the...

While wild type mice administered GDF15 lose weight, Gfral knockout mice are resistant to the effects of GDF15. Figure from Hsu, J-Y, et al1. Four independent research groups recently identified the previously orphan receptor GFRAL as the receptor for GDF15. The simultaneous publications, one in Nature1 and three in Nature Medicine2,3,4, show that GFRAL expression is restricted to brainstem regions in mice and that GDF15 acts through...

Animal models of diet-induced obesity (DIO) are commonly used to model metabolic syndrome, but have some limitations. Can a modified DIO protocol produce better type 2 diabetes models? Developing Modified DIO Protocols DIO C57BL/6 mice are obese, glucose intolerant, insulin resistant, and display mild hyperglycemia. To better model Type 2 diabetes, researchers at the University of Colorado and NYU Langone Medical Center recently developed a modified protocol...

Studying the long-term complications of diabetic pathology is more relevant than ever, driving increased interest in animal models of type 2 diabetes. According to the World Health Organization (WHO) the number of people with diabetes (includes both type 1 and type 2) has risen from 108 million in 1980 to 422 million in 2014. "[Diabetes] is a major cause of blindness, kidney failure, heart attacks, stroke, and...

Taconic Biosciences' Product Manager Jennifer Phelan recently presented a webinar on diet-induced obesity (DIO) in mouse models and the production of obese mice, including important factors in housing and husbandry. Many of your questions went unanswered due to time constraints, so we present those additional questions and answers here: Currently, Taconic does not have in-house data on the diabetic neuropathy of C57BL/6NTac DIO mice, however several publications...

A DIO (diet induced obese) mouse is a C57BL/6 - "Black 6" - mouse which has been made obese through conditioning with a special diet. These diets tend to be high in fat, however there are sometimes other modifications made such as added sugar and there are varying types of fat used to create the diet. View the Taconic Biosciences' Webinar: DIO 101: Diet-induced Obesity in Mice...