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The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
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Vnn1 exon 1 contains the translation initiation codon.
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Exon 4 has been flanked by loxP sites.
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
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Deletion of exon 4 should result in loss of function of the Vnn1 gene by deleting the C-term of Carbon-nitrogen hydrolase domain.
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The remaining recombination sites are located in intron regions of the genome.
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Data mining and Design performed in 2006.
Gene Family:
Hydrolase Genetic Background:
C57BL/6 Gene Symbol:
Vnn1 Gene Accession Number:
NM_011704 Allele Type:
KO Source:
Mouse Clinical Institute (MCI) Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
vanin 1
