Tyk2 - Model 12647 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 12647
    C57BL/6-Tyk2 tm1.1 Mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6N Tac ES cell line.
  • Tyk2 exon 3 contains the translation initiation codon.
  • Exons 4 to 6 have been flanked by loxP sites (size of loxP-flanked region: 2.3 kb).
  • The conditional KO allele has been obtained after Flp-mediated removal of the selection marker by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • Deletion of exons 4 to 6 should result in the loss of function of the Tyk2gene by deleting part of the FERM domain and by generating a frameshift from exon 3 to exons 7 to 11 (premature Stop codon in exon 7).
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • The corresponding constitutive KO allele is available as line 12684.
  • Datamining and Design performed in 2012.

Gene Family:

Kinase

Genetic Background:

C57BL/6

Gene Symbol:

Tyk2

Gene Accession Number:

NM_001205312.1

Allele Type:

cKO

Source:

TaconicArtemis

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

tyrosine kinase 2

For-profit users: Commercial users may purchase available GEM Collection models by signing the GEM Collection Commercial Terms of Sale and providing a PO. The GEM Collection Commercial Terms of Sale grant perpetual use rights.
Non-profit users: Non-profit users must provide a scientific workplan and execute an MTA prior to accessing a GEM Collection Model.

Non-profit order process is as follows:

  1. Non-profit Investigator visits the Taconic website and identifies GEM of interest.
  2. Non-profit Investigator inquires on Taconic website.
  3. Taconic will answer questions about the GEM lines and the process.
  4. Taconic sends request for MTA.
  5. Non-profit Investigator provides scientific workplan answering 4 basic questions.
  6. MTA is issued for non-profit Investigator/institution review and signature.
  7. MTA is executed by all parties and release of model is approved.
  8. Taconic contacts requestor to obtain purchase order (PO) to cover the cryorecovery and shipment costs.

Taconic begins cryorecovery upon receipt of PO and delivers model to requestor. It is the requestor's obligation to determine that the GEM meets their research needs.

Access to this model for non-profit customers requires approval of a third-party Material Transfer Agreement (MTA). The terms of this MTA are non-negotiable; an example agreement is available so that you may preview the terms. MTA approval may require 4-6 months due to circumstances outside our control. Once approved and the purchase confirmed, order fulfillment will require an additional 14-16 weeks. If you wish to continue your interest in this model, please complete the GEM Collection Inquiry Form. In addition, Taconic also offers complete model generation services that may allow for a reduced timeline to delivery of a comparable model. If you are interested in learning more, please request a GEMs Design Consultation.
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Taconic continues to strive to gain full access to all models for both non-profit and for-profit organizations. However, at this time some models are currently not available to commercial institutions. Please be sure to check back often as more models are made available on a routine basis.

Non-profit Pricing:

Price per Line ($)
$4,900

Non-profit Pricing:

Price per Line (€)
€4,459