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Tyk2 - Model 12647 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 12647
    C57BL/6-Tyk2 tm1.1 Mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6N Tac ES cell line.
  • Tyk2 exon 3 contains the translation initiation codon.
  • Exons 4 to 6 have been flanked by loxP sites (size of loxP-flanked region: 2.3 kb).
  • The conditional KO allele has been obtained after Flp-mediated removal of the selection marker by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • Deletion of exons 4 to 6 should result in the loss of function of the Tyk2gene by deleting part of the FERM domain and by generating a frameshift from exon 3 to exons 7 to 11 (premature Stop codon in exon 7).
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • The corresponding constitutive KO allele is available as line 12684.
  • Datamining and Design performed in 2012.

Gene Family:

Kinase

Genetic Background:

C57BL/6

Gene Symbol:

Tyk2

Gene Accession Number:

NM_001205312.1

Allele Type:

cKO

Source:

TaconicArtemis

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

tyrosine kinase 2

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.
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