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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exons 2 to 3 have been flanked by loxP sites (size of loxP-flanked region: 1.9 kb).
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The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
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The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
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Deletion of exons 2-3 should result in loss of function of the Slc22a1 gene by deleting two transmembrane domains (TM2 and TM3) and by generating a frameshift from exon 1 to exons 4 and 5 (premature Stop codon in exon 4).
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The remaining recombination sites are located in non-conserved regions of the genome.
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According to NCBI and Ensembl databases, the Igf2r gene(Entrez ID: 16004; ENSMUSG00000023830) is located approx. 15 kb upstream of Slc22a1 exon 2 in a tail-to-head orientation.
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The corresponding conditional KO allele is available as line 10439.
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Datamining and Design performed in 2009.
Gene Family:
Solute carrier Genetic Background:
C57BL/6 Gene Symbol:
Slc22a1 Gene Accession Number:
NM_009202.3 Allele Type:
KO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
solute carrier family 22 (organic cation transporter), member 1