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Slc16a9 - Model 10742 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 10742
    C57BL/6-Slc16a9tm1.1 mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Exon 2 contains the translation initiation codon.
  • Exons 5 and 6 have been flanked by loxP sites (size of loxP-flanked region: 4.4 kb).
  • The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 5 and 6 should result in loss of function of the Slc16a9 gene by deleting the C-terminal part of the MFS domain and the last 7 transmembrane domains, and by removing the 3´ untranslated region. The absence of 3´ UTR should result in destabilization of the Slc16a9 transcript, which is expected to be no longer expressed.
  • The remaining recombination sites are located in non-conserved regions of the genome.
  • According to NCBI and Ensembl databases, a pseudogene (ENSMUSG00000052426 ; Entrez gene# 632955) is located into 3´ UTR of the Slc16a9 gene. This pseudogene will be deleted in Slc16a9 KO allele.
  • The corresponding constitutive KO allele is available as line 11202.
  • Datamining and Design performed in 2009.

Gene Family:

Solute carrier

Genetic Background:

C57BL/6

Gene Symbol:

Slc16a9

Gene Accession Number:

NM_025807.2

Allele Type:

cKO

Source:

TaconicArtemis

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

solute carrier family 16 (monocarboxylic acid transporters), member 9

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice mutant for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.