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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 2 contains the translation initiation codon.
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Exons 5 and 6 have been flanked by loxP sites (size of loxP-flanked region: 4.4 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 5 and 6 should result in loss of function of the Slc16a9 gene by deleting the C-terminal part of the MFS domain and the last 7 transmembrane domains, and by removing the 3´ untranslated region. The absence of 3´ UTR should result in destabilization of the Slc16a9 transcript, which is expected to be no longer expressed.
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The remaining recombination sites are located in non-conserved regions of the genome.
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According to NCBI and Ensembl databases, a pseudogene (ENSMUSG00000052426 ; Entrez gene# 632955) is located into 3´ UTR of the Slc16a9 gene. This pseudogene will be deleted in Slc16a9 KO allele.
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The corresponding constitutive KO allele is available as line 11202.
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Datamining and Design performed in 2009.
Gene Family:
Solute carrier Genetic Background:
C57BL/6 Gene Symbol:
Slc16a9 Gene Accession Number:
NM_025807.2 Allele Type:
cKO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
solute carrier family 16 (monocarboxylic acid transporters), member 9
