The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 3 to 6 have been flanked by loxP sites (size of loxP-flanked region: 2.5 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 3 to 6 should result in loss of function of the Sds gene by deleting part of the catalytic domain and by generating a frameshift from exon 2 to exons 7 and 8 (premature Stop codon in exon 7).
The remaining recombination sites are located in non-conserved regions of the genome.
According to NCBI and Ensembl databases, there are 2 genes in the neighborhood of the Sds gene:
- the SdsI gene (Entrez ID: 257635; ENSMUSG00000029596) and is located approx. 7.5 kb upstream of Sds exon 3 in a head-to-head orientation.
- the 1300012G16Rik gene (Entrez ID: 71772; ENSMUSG00000029598) is located approx. 1.5 kb downstream of Sds exon 6 in a tail-to-tail orientation.
Datamining and Design performed in 2009.
Gene Family: Dehydratase
Genetic Background: C57BL/6
Gene Symbol: Sds
Gene Accession Number: NM_145565.1
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: serine dehydratase