Humanized PXR Mouse

Targeted Replacement

Humanized PXR Mouse Targeted Replacement Mouse Model
EZcohort™ Models
The easiest way to acquire and breed cryopreserved GEMs
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  • Model #
  • Genotype
  • Nomenclature
  • 9104-F
    hu/hu
    C57BL/6-Nr1i2tm1(NR1I2)Arte
  • 9104-M
    hu/hu
    C57BL/6-Nr1i2tm1(NR1I2)Arte
  • This targeted mutation strain carries a replacement of the mouse Nr1i2 gene which encodes for the nuclear receptor PXR with the corresponding human orthologous gene.
  • Useful in prediction of CYP induction and therefore pharmacokinetics, drug toxicity, and efficacy in humans.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Origin:

The Humanized PXR Mouse was developed by Taconic in collaboration with CXR Biosciences. It was created through a knock in of a human PXR cDNA/genomic construct onto the ATG of murine Pxr in C57BL/6NTac-derived ES cells. The human PXR sequence that was introduced contains a cDNA fusion of exon 2-4, intron 4, exon 5, intron 5, exon 6, intron 6, exon 7, intron 7, and cDNA fusion exon 8 + 9. The expression of the human PXR receptor is driven by the mouse Pxr promoter. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate the selection marker. Taconic received stock in 2009, and the line was embryo transfer derived. The colony is maintained by mating homozygotes.


Color:

Black

Species:

Mouse

Initial Publication:

Scheer N, Ross J, Kapelyukh Y, Rode A, Wolf CR. (2010). In vivo responses of the human and murine pregnane X receptor to dexamethasone in mice. DMD 38(7): 1046-1053.



Conditions of Use for EZcohort™ Models
EZcohort™ models are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic Biosciences, Inc. (Taconic) sells EZcohort™ models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased EZcohort™ models in consideration of purchasers' acknowledgement of and agreement to Taconic's Terms and Conditions for the Sale of Products and the following conditions of use:

  • Title to EZcohort™ models and biological materials derived from them remains with Taconic.
  • EZcohort™ models will be used for research purposes only.
  • EZcohort™ models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Breeders from deliverables can be used for propagation of EZcohort™ models or to cross to other strains.
  • Continuation of breeding and crossbreeding of EZcohort™ models outside Taconicis permitted for a term of three years post-purchase of the EZcohort™ models and is subject to annual confirmation of these EZcohort™ Conditions of Use.

Continuation of breeding and crossbreeding after the three year term will require renewal of the EZcohort™ Conditions of Use and payment of a fee.