The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 1 contains the translation initiation codon.
Exons 3 to 6 have been flanked by loxP sites (size of loxP-flanked region: 2.2 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 3 to 6 should result in loss of function of the Marc1 gene by deleting part of the MOSC domain and by generating a frameshift from exon 2 to exons 7 (premature Stop codon in exon 7).
The remaining recombination sites are located in non-conserved regions of the genome.
According to NCBI and Ensembl databases, the Mosc2 gene (Entrez ID: 67247; ENSMUSG00000073481) is located approx. 9 kb upstream of Marc1 exon 3 in a tail-to-head orientation.
The corresponding conditional KO allele is available as line 10489.
Datamining and Design performed in 2009.
- Marc1 is the current gene name. Mosc1 is an older name for this gene.
Gene Family: Sulphurase
Genetic Background: C57BL/6
Gene Symbol: Marc1
Gene Accession Number: NM_001081361.1
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: mitochondrial amidoxime reducing component 1