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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Exon 1 contains the translation initiation codon.
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Exons 5 to 7 have been flanked by loxP sites (size of loxP-flanked region: 1.5 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 5-7 should result in loss of function of the Mmab gene by deleting half of the catalytic domain and by generating a frameshift to all downstream exons.
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The remaining recombination sites are located in non-conserved regions of the genome.
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According to NCBI and Ensembl databases, the Mvk gene (NCBI ID: 17855, Ensembl: ENSMUSG00000041939) is located approx. 7 kb upstream of Mmab exon 5 in a head-to-head orientation.
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The corresponding constitutive KO allele is available as line 9984.
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Datamining and Design performed in 2008.
Gene Family:
Transferase Genetic Background:
C57BL/6 Gene Symbol:
Mmab Gene Accession Number:
NM_029956.3 Allele Type:
cKO Source:
TaconicArtemis Availability:
Cryopreserved at Taconic
Species:
Mouse Official Gene Name:
methylmalonic aciduria (cobalamin deficiency) type B homolog (human)
