The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
Mcart1 exon 2 contains the translation initiation codon.
Exon 3 has been flanked by loxP sites in 3’UTR region.
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
Deletion of exon 3 should result in loss of function of the Mcart1 gene by deleting part of the protein and stop codon in exon 3.
The remaining recombination sites are located in intron regions of the genome.
Datamining and Design performed in 2008.
Gene Family: Mitochondrial carrier
Genetic Background: C57BL/6
Gene Symbol: Mcart1
Gene Accession Number: NM_001009949
Allele Type: KO
Source: Institut Clinique de la Souris (ICS), Illkirch, France
Availability: Cryopreserved at Taconic
Official Gene Name: mitochondrial carrier triple repeat 1