The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Exon 2 contains the translation initiation codon.
Exons 4 to 7 have been flanked by loxP sites (size of loxP-flanked region: 2.9 kb).
The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
Deletion of exons 4 to 7 should result in loss of function of the Lpin3 gene by deleting the C-terminal part of the Lipin domain and the potential nuclear localization signal and by generating a frameshift from exon 3 to exons 8-11 (premature Stop codon in exon 8).
The remaining recombination sites are located in non-conserved regions of the genome.
According to NCBI and Ensembl databases, there are two genes in the neighborhood of the Lpin3 gene:
- The Zhx3 gene (Entrez ID: 320799; ENSMUSG00000035877) is located approx. 22 kb upstream of Lpin3 exon 4 in a head-to-head orientation.
- The Emilin3 gene (Entrez ID: 280635; ENSMUSG00000050700) is located approx. 9 kb downstream of Lpin3 exon 7 in a tail-to-tail orientation.
The corresponding conditional KO allele is available as line 10584.
Datamining and Design performed in 2009.
Gene Family: Phosphatase
Genetic Background: C57BL/6
Gene Symbol: Lpin3
Gene Accession Number: NM_022883.2
Allele Type: KO
Availability: Cryopreserved at Taconic
Official Gene Name: lipin 3