A BAC containing the mouse Grn gene was engineered a ~30 kb DNA region encompassing the Grn promoter and exons 1-13, but not the neighboring Rundc3a, Slc25a39, and Itga2b genes.
The BAC construct was microinjected in C57BL6 mice by pronuclear injection.
Putative transgenic founders were identified by genotyping and bred for germ line transmission with C57BL/6 mice.
Heterozygous transgenic mice were identified by genotyping.
Line 10015, C57BL/6-Tg(Grn)42 Mrl, has the lowest expression level of the BAC transgene in the brain (2 fold at mRNA level).
Line 10016, C57BL/6-Tg(Grn)33 Mrl, has second highest expression level of the BAC transgene in the brain (5-7 fold at mRNA level).
Line 10150, C57BL/6-Tg(Grn)26 Mrl, has the highest expression level of the BAC transgene in the brain (10-14 fold at mRNA level).
Datamining and Design performed in 2008.
Gene Family: Growth factor
Genetic Background: C57BL/6
Gene Symbol: Grn
Gene Accession Number: NM_008175
Allele Type: TG
Availability: Cryopreserved at Taconic
Official Gene Name: granulin