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The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
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Gpam exon 2 contains the translation initiation site.
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Exons 9 and 10 have been flanked by LoxP sites (size of floxed region 1.5 kb).
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The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
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The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
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The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 9 and 10 should result in loss of function of the Gpam gene by deleting part of the acetyl-transferase domain and by generating a frameshift from exon 8 to exons 11 and 12.
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Remaining recombination sites will be located in non-conserved regions of the genome.
- The constitutive KO allele is available as line 10910.
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Datamining and Design performed in 2009.
Gene Family:
Transferase Genetic Background:
C57BL/6 Gene Symbol:
Gpam Gene Accession Number:
NM_008149 Allele Type:
cKO Source:
Merck Research Laboratories Availability:
Cryopreserved at Taconic Species:
Mouse Official Gene Name:
glycerol-3-phosphate acyltransferase, mitochondrialprovided