Read Taconic's response to coronavirus (COVID-19), and visit our toolkit for researchers. Learn More

Gpam - Model 11116 - cKO

Conditional Knockout

Cryopreserved Model
This line is cryopreserved and requires cryorecovery.

C57BL/6 Background

  • Model #
  • Genotype
  • Nomenclature
  • 11116
    C57BL/6-Gpamtm1.1 Mrl
  • The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
  • Gpam exon 2 contains the translation initiation site.
  • Exons 9 and 10 have been flanked by LoxP sites (size of floxed region 1.5 kb).
  • The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
  • The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
  • The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 9 and 10 should result in loss of function of the Gpam gene by deleting part of the acetyl-transferase domain and by generating a frameshift from exon 8 to exons 11 and 12.
  • Remaining recombination sites will be located in non-conserved regions of the genome.
  • The constitutive KO allele is available as line 10910.
  • Datamining and Design performed in 2009.

Gene Family:

Transferase

Genetic Background:

C57BL/6

Gene Symbol:

Gpam

Gene Accession Number:

NM_008149

Allele Type:

cKO

Source:

Merck Research Laboratories

Availability:

Cryopreserved at Taconic

Species:

Mouse

Official Gene Name:

glycerol-3-phosphate acyltransferase, mitochondrialprovided

For Profit Pricing:

Price per Line ($)
$30,000

Nonprofit Pricing:

Price per Line ($)
$5,500

For Profit Pricing:

Price per Line (€)
€27,300

Nonprofit Pricing:

Price per Line (€)
€5,005
GEM Collection models exist as cryopreserved materials. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Customers will receive:

  • A minimum of four (4) mice heterozygous for the selected mutation. In rare cases where the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 heterozygous mice.
  • Validated genotyping protocols
  • Animal health report. Mice will meet Taconic's Murine Pathogen Free™ (MPF™) health standard.

Please note that in rare instances (2%) cryorecovery may prove to be difficult, either extending timeline or ultimately successful recovery.
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO. The GEM Collection Commercial and Academic Terms of Sale grant perpetual use rights.