The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
Gpam exon 2 contains the translation initiation site.
Exons 9 and 10 have been flanked by LoxP sites (size of floxed region 1.5 kb).
The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 9 and 10 should result in loss of function of the Gpam gene by deleting part of the acetyl-transferase domain and by generating a frameshift from exon 8 to exons 11 and 12.
Remaining recombination sites will be located in non-conserved regions of the genome.
- The constitutive KO allele is available as line 10910.
Datamining and Design performed in 2009.
Gene Family: Transferase
Genetic Background: C57BL/6
Gene Symbol: Gpam
Gene Accession Number: NM_008149
Allele Type: cKO
Source: Merck Research Laboratories
Availability: Cryopreserved at Taconic
Official Gene Name: glycerol-3-phosphate acyltransferase, mitochondrialprovided